AgrobacTransV1, Main, Exploration, bibRecord, 000608

Effect of codon optimization and subcellular targeting on Toxoplasma gondii antigen SAG1 expression in tobacco leaves to use in subcutaneous and oral immunization in mice.

Identifieur interne : 000608 ( Main/Exploration ); précédent : 000607; suivant : 000609

Effect of codon optimization and subcellular targeting on Toxoplasma gondii antigen SAG1 expression in tobacco leaves to use in subcutaneous and oral immunization in mice.

Auteurs : Melina Laguía-Becher [Argentine] ; Valentina Martín ; Mauricio Kraemer ; Mariana Corigliano ; María L. Yacono ; Alejandra Goldman ; Marina Clemente

Source :

BMC biotechnology [ 1472-6750 ] ; 2010.

RBID : pubmed:20633272

Descripteurs français

English descriptors

KwdEn :
- Animals (MeSH), Antigens, Protozoan (immunology), Base Sequence (MeSH), Codon (genetics), Female (MeSH), Immunity, Cellular (MeSH), Immunity, Humoral (MeSH), Immunization (methods), Interferon-gamma (immunology), Mice (MeSH), Mice, Inbred C3H (MeSH), Mice, Inbred C57BL (MeSH), Molecular Sequence Data (MeSH), Plant Leaves (genetics), Plant Leaves (immunology), Plant Leaves (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (immunology), Protozoan Proteins (immunology), Protozoan Vaccines (immunology), Recombinant Proteins (immunology), Th1 Cells (immunology), Tobacco (genetics), Tobacco (immunology), Tobacco (metabolism), Toxoplasma (immunology), Toxoplasmosis, Animal (immunology), Toxoplasmosis, Animal (prevention & control).
MESH :
- chemical , genetics : Codon.
- chemical , immunology : Antigens, Protozoan, Interferon-gamma, Protozoan Proteins, Protozoan Vaccines, Recombinant Proteins.
- genetics : Plant Leaves, Plants, Genetically Modified, Tobacco.
- immunology : Plant Leaves, Plants, Genetically Modified, Th1 Cells, Tobacco, Toxoplasma, Toxoplasmosis, Animal.
- metabolism : Plant Leaves, Tobacco.
- methods : Immunization.
- prevention & control : Toxoplasmosis, Animal.
- Animals, Base Sequence, Female, Immunity, Cellular, Immunity, Humoral, Mice, Mice, Inbred C3H, Mice, Inbred C57BL, Molecular Sequence Data.

Abstract

BACKGROUND

Codon optimization and subcellular targeting were studied with the aim to increase the expression levels of the SAG178-322 antigen of Toxoplasma gondii in tobacco leaves. The expression of the tobacco-optimized and native versions of the SAG1 gene was explored by transient expression from the Agrobacterium tumefaciens binary expression vector, which allows targeting the recombinant protein to the endoplasmic reticulum (ER) and the apoplast. Finally, mice were subcutaneously and orally immunized with leaf extracts-SAG1 and the strategy of prime boost with rSAG1 expressed in Escherichia coli was used to optimize the oral immunization with leaf extracts-SAG1.

RESULTS

Leaves agroinfiltrated with an unmodified SAG1 gene accumulated 5- to 10-fold more than leaves agroinfiltrated with a codon-optimized SAG1 gene. ER localization allowed the accumulation of higher levels of native SAG1. However, no significant differences were observed between the mRNA accumulations of the different versions of SAG1. Subcutaneous immunization with leaf extracts-SAG1 (SAG1) protected mice against an oral challenge with a non-lethal cyst dose, and this effect could be associated with the secretion of significant levels of IFN-gamma. The protection was increased when mice were ID boosted with rSAG1 (SAG1+boost). This group elicited a significant Th1 humoral and cellular immune response characterized by high levels of IFN-gamma. In an oral immunization assay, the SAG1+boost group showed a significantly lower brain cyst burden compared to the rest of the groups.

CONCLUSION

Transient agroinfiltration was useful for the expression of all of the recombinant proteins tested. Our results support the usefulness of endoplasmic reticulum signal peptides in enhancing the production of recombinant proteins meant for use as vaccines. The results showed that this plant-produced protein has potential for use as vaccine and provides a potential means for protecting humans and animals against toxoplasmosis.

DOI: 10.1186/1472-6750-10-52
PubMed: 20633272
PubMed Central: PMC2920232

Affiliations:

Argentine

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000596
to stream Main, to step Curation: 000596

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Effect of codon optimization and subcellular targeting on Toxoplasma gondii antigen SAG1 expression in tobacco leaves to use in subcutaneous and oral immunization in mice.</title>
<author><name sortKey="Laguia Becher, Melina" sort="Laguia Becher, Melina" uniqKey="Laguia Becher M" first="Melina" last="Laguía-Becher">Melina Laguía-Becher</name>
<affiliation wicri:level="1"><nlm:affiliation>IIB-INTECH, Camino de Circunvalación km 6, Provincia de Buenos Aires, Argentina.</nlm:affiliation>
<country xml:lang="fr">Argentine</country>
<wicri:regionArea>IIB-INTECH, Camino de Circunvalación km 6, Provincia de Buenos Aires</wicri:regionArea>
<wicri:noRegion>Provincia de Buenos Aires</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Martin, Valentina" sort="Martin, Valentina" uniqKey="Martin V" first="Valentina" last="Martín">Valentina Martín</name>
</author>
<author><name sortKey="Kraemer, Mauricio" sort="Kraemer, Mauricio" uniqKey="Kraemer M" first="Mauricio" last="Kraemer">Mauricio Kraemer</name>
</author>
<author><name sortKey="Corigliano, Mariana" sort="Corigliano, Mariana" uniqKey="Corigliano M" first="Mariana" last="Corigliano">Mariana Corigliano</name>
</author>
<author><name sortKey="Yacono, Maria L" sort="Yacono, Maria L" uniqKey="Yacono M" first="María L" last="Yacono">María L. Yacono</name>
</author>
<author><name sortKey="Goldman, Alejandra" sort="Goldman, Alejandra" uniqKey="Goldman A" first="Alejandra" last="Goldman">Alejandra Goldman</name>
</author>
<author><name sortKey="Clemente, Marina" sort="Clemente, Marina" uniqKey="Clemente M" first="Marina" last="Clemente">Marina Clemente</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2010">2010</date>
<idno type="RBID">pubmed:20633272</idno>
<idno type="pmid">20633272</idno>
<idno type="doi">10.1186/1472-6750-10-52</idno>
<idno type="pmc">PMC2920232</idno>
<idno type="wicri:Area/Main/Corpus">000596</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000596</idno>
<idno type="wicri:Area/Main/Curation">000596</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000596</idno>
<idno type="wicri:Area/Main/Exploration">000596</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Effect of codon optimization and subcellular targeting on Toxoplasma gondii antigen SAG1 expression in tobacco leaves to use in subcutaneous and oral immunization in mice.</title>
<author><name sortKey="Laguia Becher, Melina" sort="Laguia Becher, Melina" uniqKey="Laguia Becher M" first="Melina" last="Laguía-Becher">Melina Laguía-Becher</name>
<affiliation wicri:level="1"><nlm:affiliation>IIB-INTECH, Camino de Circunvalación km 6, Provincia de Buenos Aires, Argentina.</nlm:affiliation>
<country xml:lang="fr">Argentine</country>
<wicri:regionArea>IIB-INTECH, Camino de Circunvalación km 6, Provincia de Buenos Aires</wicri:regionArea>
<wicri:noRegion>Provincia de Buenos Aires</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Martin, Valentina" sort="Martin, Valentina" uniqKey="Martin V" first="Valentina" last="Martín">Valentina Martín</name>
</author>
<author><name sortKey="Kraemer, Mauricio" sort="Kraemer, Mauricio" uniqKey="Kraemer M" first="Mauricio" last="Kraemer">Mauricio Kraemer</name>
</author>
<author><name sortKey="Corigliano, Mariana" sort="Corigliano, Mariana" uniqKey="Corigliano M" first="Mariana" last="Corigliano">Mariana Corigliano</name>
</author>
<author><name sortKey="Yacono, Maria L" sort="Yacono, Maria L" uniqKey="Yacono M" first="María L" last="Yacono">María L. Yacono</name>
</author>
<author><name sortKey="Goldman, Alejandra" sort="Goldman, Alejandra" uniqKey="Goldman A" first="Alejandra" last="Goldman">Alejandra Goldman</name>
</author>
<author><name sortKey="Clemente, Marina" sort="Clemente, Marina" uniqKey="Clemente M" first="Marina" last="Clemente">Marina Clemente</name>
</author>
</analytic>
<series><title level="j">BMC biotechnology</title>
<idno type="eISSN">1472-6750</idno>
<imprint><date when="2010" type="published">2010</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term>
<term>Antigens, Protozoan (immunology)</term>
<term>Base Sequence (MeSH)</term>
<term>Codon (genetics)</term>
<term>Female (MeSH)</term>
<term>Immunity, Cellular (MeSH)</term>
<term>Immunity, Humoral (MeSH)</term>
<term>Immunization (methods)</term>
<term>Interferon-gamma (immunology)</term>
<term>Mice (MeSH)</term>
<term>Mice, Inbred C3H (MeSH)</term>
<term>Mice, Inbred C57BL (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Plant Leaves (genetics)</term>
<term>Plant Leaves (immunology)</term>
<term>Plant Leaves (metabolism)</term>
<term>Plants, Genetically Modified (genetics)</term>
<term>Plants, Genetically Modified (immunology)</term>
<term>Protozoan Proteins (immunology)</term>
<term>Protozoan Vaccines (immunology)</term>
<term>Recombinant Proteins (immunology)</term>
<term>Th1 Cells (immunology)</term>
<term>Tobacco (genetics)</term>
<term>Tobacco (immunology)</term>
<term>Tobacco (metabolism)</term>
<term>Toxoplasma (immunology)</term>
<term>Toxoplasmosis, Animal (immunology)</term>
<term>Toxoplasmosis, Animal (prevention & control)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term>
<term>Antigènes de protozoaire (immunologie)</term>
<term>Codon (génétique)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Femelle (MeSH)</term>
<term>Feuilles de plante (génétique)</term>
<term>Feuilles de plante (immunologie)</term>
<term>Feuilles de plante (métabolisme)</term>
<term>Immunisation (méthodes)</term>
<term>Immunité cellulaire (MeSH)</term>
<term>Immunité humorale (MeSH)</term>
<term>Interféron gamma (immunologie)</term>
<term>Lymphocytes auxiliaires Th1 (immunologie)</term>
<term>Protéines de protozoaire (immunologie)</term>
<term>Protéines recombinantes (immunologie)</term>
<term>Souris (MeSH)</term>
<term>Souris de lignée C3H (MeSH)</term>
<term>Souris de lignée C57BL (MeSH)</term>
<term>Séquence nucléotidique (MeSH)</term>
<term>Tabac (génétique)</term>
<term>Tabac (immunologie)</term>
<term>Tabac (métabolisme)</term>
<term>Toxoplasma (immunologie)</term>
<term>Toxoplasmose animale (immunologie)</term>
<term>Toxoplasmose animale (prévention et contrôle)</term>
<term>Vaccins antiprotozoaires (immunologie)</term>
<term>Végétaux génétiquement modifiés (génétique)</term>
<term>Végétaux génétiquement modifiés (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Codon</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Protozoan</term>
<term>Interferon-gamma</term>
<term>Protozoan Proteins</term>
<term>Protozoan Vaccines</term>
<term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Leaves</term>
<term>Plants, Genetically Modified</term>
<term>Tobacco</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Codon</term>
<term>Feuilles de plante</term>
<term>Tabac</term>
<term>Végétaux génétiquement modifiés</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes de protozoaire</term>
<term>Feuilles de plante</term>
<term>Interféron gamma</term>
<term>Lymphocytes auxiliaires Th1</term>
<term>Protéines de protozoaire</term>
<term>Protéines recombinantes</term>
<term>Tabac</term>
<term>Toxoplasma</term>
<term>Toxoplasmose animale</term>
<term>Vaccins antiprotozoaires</term>
<term>Végétaux génétiquement modifiés</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Plant Leaves</term>
<term>Plants, Genetically Modified</term>
<term>Th1 Cells</term>
<term>Tobacco</term>
<term>Toxoplasma</term>
<term>Toxoplasmosis, Animal</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plant Leaves</term>
<term>Tobacco</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Immunization</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Feuilles de plante</term>
<term>Tabac</term>
</keywords>
<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Immunisation</term>
</keywords>
<keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Toxoplasmosis, Animal</term>
</keywords>
<keywords scheme="MESH" qualifier="prévention et contrôle" xml:lang="fr"><term>Toxoplasmose animale</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Base Sequence</term>
<term>Female</term>
<term>Immunity, Cellular</term>
<term>Immunity, Humoral</term>
<term>Mice</term>
<term>Mice, Inbred C3H</term>
<term>Mice, Inbred C57BL</term>
<term>Molecular Sequence Data</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Données de séquences moléculaires</term>
<term>Femelle</term>
<term>Immunité cellulaire</term>
<term>Immunité humorale</term>
<term>Souris</term>
<term>Souris de lignée C3H</term>
<term>Souris de lignée C57BL</term>
<term>Séquence nucléotidique</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b>
</p>
<p>Codon optimization and subcellular targeting were studied with the aim to increase the expression levels of the SAG178-322 antigen of Toxoplasma gondii in tobacco leaves. The expression of the tobacco-optimized and native versions of the SAG1 gene was explored by transient expression from the Agrobacterium tumefaciens binary expression vector, which allows targeting the recombinant protein to the endoplasmic reticulum (ER) and the apoplast. Finally, mice were subcutaneously and orally immunized with leaf extracts-SAG1 and the strategy of prime boost with rSAG1 expressed in Escherichia coli was used to optimize the oral immunization with leaf extracts-SAG1.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>RESULTS</b>
</p>
<p>Leaves agroinfiltrated with an unmodified SAG1 gene accumulated 5- to 10-fold more than leaves agroinfiltrated with a codon-optimized SAG1 gene. ER localization allowed the accumulation of higher levels of native SAG1. However, no significant differences were observed between the mRNA accumulations of the different versions of SAG1. Subcutaneous immunization with leaf extracts-SAG1 (SAG1) protected mice against an oral challenge with a non-lethal cyst dose, and this effect could be associated with the secretion of significant levels of IFN-gamma. The protection was increased when mice were ID boosted with rSAG1 (SAG1+boost). This group elicited a significant Th1 humoral and cellular immune response characterized by high levels of IFN-gamma. In an oral immunization assay, the SAG1+boost group showed a significantly lower brain cyst burden compared to the rest of the groups.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>CONCLUSION</b>
</p>
<p>Transient agroinfiltration was useful for the expression of all of the recombinant proteins tested. Our results support the usefulness of endoplasmic reticulum signal peptides in enhancing the production of recombinant proteins meant for use as vaccines. The results showed that this plant-produced protein has potential for use as vaccine and provides a potential means for protecting humans and animals against toxoplasmosis.</p>
</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20633272</PMID>
<DateCompleted><Year>2010</Year>
<Month>10</Month>
<Day>15</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1472-6750</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>10</Volume>
<PubDate><Year>2010</Year>
<Month>Jul</Month>
<Day>15</Day>
</PubDate>
</JournalIssue>
<Title>BMC biotechnology</Title>
<ISOAbbreviation>BMC Biotechnol</ISOAbbreviation>
</Journal>
<ArticleTitle>Effect of codon optimization and subcellular targeting on Toxoplasma gondii antigen SAG1 expression in tobacco leaves to use in subcutaneous and oral immunization in mice.</ArticleTitle>
<Pagination><MedlinePgn>52</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1186/1472-6750-10-52</ELocationID>
<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Codon optimization and subcellular targeting were studied with the aim to increase the expression levels of the SAG178-322 antigen of Toxoplasma gondii in tobacco leaves. The expression of the tobacco-optimized and native versions of the SAG1 gene was explored by transient expression from the Agrobacterium tumefaciens binary expression vector, which allows targeting the recombinant protein to the endoplasmic reticulum (ER) and the apoplast. Finally, mice were subcutaneously and orally immunized with leaf extracts-SAG1 and the strategy of prime boost with rSAG1 expressed in Escherichia coli was used to optimize the oral immunization with leaf extracts-SAG1.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">Leaves agroinfiltrated with an unmodified SAG1 gene accumulated 5- to 10-fold more than leaves agroinfiltrated with a codon-optimized SAG1 gene. ER localization allowed the accumulation of higher levels of native SAG1. However, no significant differences were observed between the mRNA accumulations of the different versions of SAG1. Subcutaneous immunization with leaf extracts-SAG1 (SAG1) protected mice against an oral challenge with a non-lethal cyst dose, and this effect could be associated with the secretion of significant levels of IFN-gamma. The protection was increased when mice were ID boosted with rSAG1 (SAG1+boost). This group elicited a significant Th1 humoral and cellular immune response characterized by high levels of IFN-gamma. In an oral immunization assay, the SAG1+boost group showed a significantly lower brain cyst burden compared to the rest of the groups.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">Transient agroinfiltration was useful for the expression of all of the recombinant proteins tested. Our results support the usefulness of endoplasmic reticulum signal peptides in enhancing the production of recombinant proteins meant for use as vaccines. The results showed that this plant-produced protein has potential for use as vaccine and provides a potential means for protecting humans and animals against toxoplasmosis.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Laguía-Becher</LastName>
<ForeName>Melina</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>IIB-INTECH, Camino de Circunvalación km 6, Provincia de Buenos Aires, Argentina.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Martín</LastName>
<ForeName>Valentina</ForeName>
<Initials>V</Initials>
</Author>
<Author ValidYN="Y"><LastName>Kraemer</LastName>
<ForeName>Mauricio</ForeName>
<Initials>M</Initials>
</Author>
<Author ValidYN="Y"><LastName>Corigliano</LastName>
<ForeName>Mariana</ForeName>
<Initials>M</Initials>
</Author>
<Author ValidYN="Y"><LastName>Yacono</LastName>
<ForeName>María L</ForeName>
<Initials>ML</Initials>
</Author>
<Author ValidYN="Y"><LastName>Goldman</LastName>
<ForeName>Alejandra</ForeName>
<Initials>A</Initials>
</Author>
<Author ValidYN="Y"><LastName>Clemente</LastName>
<ForeName>Marina</ForeName>
<Initials>M</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2010</Year>
<Month>07</Month>
<Day>15</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>BMC Biotechnol</MedlineTA>
<NlmUniqueID>101088663</NlmUniqueID>
<ISSNLinking>1472-6750</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000953">Antigens, Protozoan</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D003062">Codon</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D015800">Protozoan Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D016052">Protozoan Vaccines</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C047168">SAG1 antigen, Toxoplasma</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>82115-62-6</RegistryNumber>
<NameOfSubstance UI="D007371">Interferon-gamma</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000953" MajorTopicYN="N">Antigens, Protozoan</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003062" MajorTopicYN="N">Codon</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007111" MajorTopicYN="N">Immunity, Cellular</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D056724" MajorTopicYN="N">Immunity, Humoral</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007114" MajorTopicYN="N">Immunization</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007371" MajorTopicYN="N">Interferon-gamma</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008809" MajorTopicYN="N">Mice, Inbred C3H</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008810" MajorTopicYN="N">Mice, Inbred C57BL</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015800" MajorTopicYN="N">Protozoan Proteins</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016052" MajorTopicYN="N">Protozoan Vaccines</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018417" MajorTopicYN="N">Th1 Cells</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014122" MajorTopicYN="N">Toxoplasma</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014124" MajorTopicYN="N">Toxoplasmosis, Animal</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000517" MajorTopicYN="Y">prevention & control</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2009</Year>
<Month>12</Month>
<Day>21</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2010</Year>
<Month>07</Month>
<Day>15</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2010</Year>
<Month>7</Month>
<Day>17</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2010</Year>
<Month>7</Month>
<Day>17</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2010</Year>
<Month>10</Month>
<Day>16</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>epublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">20633272</ArticleId>
<ArticleId IdType="pii">1472-6750-10-52</ArticleId>
<ArticleId IdType="doi">10.1186/1472-6750-10-52</ArticleId>
<ArticleId IdType="pmc">PMC2920232</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>J Mol Biol. 2005 Nov 25;354(2):447-58</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16242717</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int J Med Microbiol. 2008 Jan;298(1-2):39-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17716944</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1987 Feb 11;15(3):1281-95</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3547335</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vaccine. 1998 Aug;16(13):1283-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9682392</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Infect Dis. 2000 Jan;181(1):317-24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10608781</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int J Parasitol. 2008 Jan;38(1):103-10</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17681344</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Transgenic Res. 2007 Jun;16(3):315-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17436059</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Infect Dis. 1992 Aug;15(2):211-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1520757</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Biotechnol. 2002 Jun;20(6):622-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12042869</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Rep. 2006 Jul;25(7):689-97</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16491379</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2003 Mar;33(5):949-56</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12609035</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Plant Sci. 1999 Nov;4(11):429-438</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10529824</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vaccine. 2009 Jul 30;27(35):4798-807</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19539581</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Diagn Lab Immunol. 1996 May;3(3):355-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8705683</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 1992 Mar;2(2):181-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1302048</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Neurochem Res. 2002 Oct;27(10):957-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12462398</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Immunol Res. 2003;27(2-3):521-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12857995</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Exp Parasitol. 2008 Sep;120(1):118-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18588877</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vaccine. 2008 Aug 18;26(35):4541-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18590785</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Reprod Toxicol. 2006 May;21(4):458-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16311017</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Biotechnol. 2007;7:27</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17537261</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Biotechnol J. 2007 Jan;5(1):2-15</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17207252</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 1989 Apr 5;264(10):5569-74</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2925621</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Transgenic Res. 2006 Aug;15(4):455-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16906446</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Expert Rev Vaccines. 2008 Oct;7(8):1173-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18844592</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vet Pathol. 1990 Nov;27(6):411-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2278129</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Biotechnol J. 2009 Dec;7(9):846-55</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19781007</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vet Res. 1998 May-Aug;29(3-4):289-310</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9689743</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2004 Nov 26;279(48):49919-30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15347681</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int J Parasitol. 2000 Nov;30(12-13):1217-58</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11113252</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11539-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11553782</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Biotechnol. 2006 Sep;24(9):426-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16843560</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Drug Targets Immune Endocr Metabol Disord. 2002 Oct;2(3):227-34</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12476487</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2005 Nov;44(3):471-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16236156</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vaccine. 2009 Mar 4;27(10):1596-600</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19146896</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Immunol. 1992 Mar 1;148(5):1493-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1538132</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2005 Sep 1;339(2):153-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15992851</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 1988 Oct 25;16(20):9877</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3186459</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Infect Immun. 1998 Sep;66(9):4503-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9712808</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cancer Res. 2002 Jul 1;62(13):3654-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12097270</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vaccine. 2003 Jun 20;21(21-22):2813-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12798622</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Immunobiology. 2008;213(8):671-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18950595</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Infect Immun. 1999 Dec;67(12):6358-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10569750</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Microbes Infect. 2003 Apr;5(5):457-62</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12738002</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Biotechnol. 2008;8:53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18573204</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biotechnol. 2005 May;30(1):41-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15805575</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 1998 Aug;117(4):1445-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9701600</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Schweiz Arch Tierheilkd. 2000 Mar;142(3):95-108</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10748708</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Gen Virol. 2007 May;88(Pt 5):1460-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17412974</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Vaccine. 2008 Mar 28;26(15):1846-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18325641</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Transgenic Res. 2009 Apr;18(2):185-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18763047</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Immunol. 2005 Sep 1;175(5):2777-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16116161</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Parasitol Res. 2007 Mar;100(4):829-35</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17058109</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Infect Immun. 2000 Sep;68(9):4948-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10948109</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Hum Gene Ther. 2006 Apr;17(4):415-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16610929</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Chin Med J (Engl). 2002 Oct;115(10):1448-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12490084</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Argentine</li>
</country>
</list>
<tree><noCountry><name sortKey="Clemente, Marina" sort="Clemente, Marina" uniqKey="Clemente M" first="Marina" last="Clemente">Marina Clemente</name>
<name sortKey="Corigliano, Mariana" sort="Corigliano, Mariana" uniqKey="Corigliano M" first="Mariana" last="Corigliano">Mariana Corigliano</name>
<name sortKey="Goldman, Alejandra" sort="Goldman, Alejandra" uniqKey="Goldman A" first="Alejandra" last="Goldman">Alejandra Goldman</name>
<name sortKey="Kraemer, Mauricio" sort="Kraemer, Mauricio" uniqKey="Kraemer M" first="Mauricio" last="Kraemer">Mauricio Kraemer</name>
<name sortKey="Martin, Valentina" sort="Martin, Valentina" uniqKey="Martin V" first="Valentina" last="Martín">Valentina Martín</name>
<name sortKey="Yacono, Maria L" sort="Yacono, Maria L" uniqKey="Yacono M" first="María L" last="Yacono">María L. Yacono</name>
</noCountry>
<country name="Argentine"><noRegion><name sortKey="Laguia Becher, Melina" sort="Laguia Becher, Melina" uniqKey="Laguia Becher M" first="Melina" last="Laguía-Becher">Melina Laguía-Becher</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/AgrobacTransV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000608 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000608 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    AgrobacTransV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:20633272
   |texte=   Effect of codon optimization and subcellular targeting on Toxoplasma gondii antigen SAG1 expression in tobacco leaves to use in subcutaneous and oral immunization in mice.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:20633272" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a AgrobacTransV1

This area was generated with Dilib version V0.6.38.
Data generation: Fri Nov 20 15:45:55 2020. Site generation: Wed Mar 6 15:24:41 2024

	Serveur d'exploration sur l'agrobacterium et la transgénèse
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur l'agrobacterium et la transgénèse

Effect of codon optimization and subcellular targeting on Toxoplasma gondii antigen SAG1 expression in tobacco leaves to use in subcutaneous and oral immunization in mice.

Effect of codon optimization and subcellular targeting on Toxoplasma gondii antigen SAG1 expression in tobacco leaves to use in subcutaneous and oral immunization in mice.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki